Pathological changes of vascular tissue after rabbit limb replantation with different methods of cryopreservation

doi:10.3969/j.issn.2095-4344.2014.15.011

Abstract

Abstract:

BACKGROUND: The cryopreservation of single tissue has achieved great advancement and is gradually applied in clinics. However, the cryopreservation of complex tissue is rarely reported.
OBJECTIVE: To investigate the morphological change in rabbit limb tissue after replantation through different rewarming methods, find the best rewarming methods of compound textured blood vessels, and provide theoretical basis for the feasibility of limb replantation after long-term cryopreservation.
METHODS: Thirty New Zealand white rabbits were randomly divided into control group, slow freezing-slow thawing group, and slow freezing-rapid thawing group. The right posterior limbs of all the rabbits were cut off 1 cm above the knee joint. Except control group, the latter two groups were given limb replantation after thawing, and then the right posterior limb was again cut off after the replanted limbs were survived for 6 hours. For all groups, the histological changes and gross observation in aorta tissue were observed by light microscopy and transmission electron microscope, and the results were analyzed with statistical methods.
RESULTS AND CONCLUSION: In the slow freezing-slow thawing, slow freezing-rapid thawing groups, the pathological changes (gross specimen, light microscope, electron microscope) of rabbit limbs 6 hours after replantation were worse than those in control group. Compared with slow freezing-rapid thawing group, better integrity of endothelial cells and less damage of the organelles were found in slow freezing-slow thawing group. Through deep cryogenic freezing-thawing process, rabbit limb blood vessels can maintain the structural integrity after replantation and survived at 6 hours. Slow freezing-slow thawing is better than slow freezing-rapid thawing for the preservation of severed limbs, providing evidences for the long-term survival following a deep cry ogenic treatment after the severed limb replantation.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: cryopreservation, hindlimb, transplantation, disarticulation, blood vessels, endothelial cells

CLC Number:

R318

Li Bo, He Jian-ping, Zhang Shu-ming, Zhu Ze-xing, Qiao Lin, Qiao Ya-nan. Pathological changes of vascular tissue after rabbit limb replantation with different methods of cryopreservation [J]. Chinese Journal of Tissue Engineering Research, 2014, 18(15): 2357-2362.

Figures/Tables 3

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